Methylene Blue - clinical uses

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[THE DETAILS ON THIS PAGE ARE COVERED IN MORE DETAIL IN Diagnosis and Treatment of Chronic Fatigue Syndrome, Myalgic Encephalitis and Long-Covid (3rd Edition) ]


for energy and inflammation

Methylene blue (MB) has some properties that make it very desirable for the human body. It is remarkably safe and has been used in humans for decades. This makes it very undesirable for Big Pharma, so its properties are not reported to the medical profession.

There are two major drivers of disease namely poor energy delivery mechanisms and chronic inflammation. MB impacts on both. A further problem arises because as we become ill, the immune system starts to fail, and we acquire not just one but multiple infections. Methylene blue has activity against many infections (viral, bacterial and fungal), but what is so interesting is that its effects are activated by red light. See, as just one example of many studies, Methylene blue photodynamic therapy induces selective and massive cell death in human breast cancer cells and also see below.

MB could be further combined with Dimethylsulphoxide (DMSO) as this too has anti-microbial effects possibly through its effect on biofilm. See Dimethylsulphoxide (DMSO) – another multitasking, inexpensive, safe and effective tool.

So, to tackle any chronic infections - start with Groundhog CHRONIC (PK diet, sort the gut, supplements, detox, sort the thyroid and adrenal glands) - see Groundhog CHRONIC – the starting point to treat any chronic infection AND to live to our full potential

THEN add in MB plus DMSO, both of which are activated by red light.

Start with low doses of MB, add in low doses of DMSO then add in red light. Build up very slowly to mitigate DDD reactions - see Diet, Detox and Die-off Reactions Expect to get worse

See Dimethylsulphoxide (DMSO) – another multitasking, inexpensive, safe and effective tool for doses of DMSO, and see below for doses of MB.

MB is a monoamine oxidase inhibitor which explains its anti-depressive and anti-anxiety effects - see Methylene blue and its analogues as antidepressant compounds

However, you MUST avoid foods that contain tyramine (cheese, soy sauce, liver, alcohol and salami).

Also, avoid prescription medications such as SSRIs and tricyclic antidepressants.

MAOIs can cause serious reactions when you take them with certain medications, such as antidepressants (including also SNRIs), certain pain drugs, certain cold and allergy medications, amphetamines, blood pressure drugs, migraine drugs, some antibiotics or antifungals, recreational drugs, Tegretol, disulphiram and some herbal supplements such as St John’s Wort or ginseng.

If you are taking ANY medication, do check for interactions with MAOIs. See Serious Risks with MAO Inhibitors

The French national library (Bibliothèque Nationale de France)

The French national library (Bibliothèque Nationale de France) has over a hundred references on MB some dating back to 1891.

I am indedted to Guy Eschemann, member of my Facebook Group, who notified me of this.

See French National Library Entries on Methylene Blue

Desirable properties of MB

Action Disease References
MB improves mitochondrial function and so energy delivery mechanisms. MB can also trap leaking electrons produced by mitochondrial inhibitors and preserve the metabolic rate by bypassing blocked points of electron flow, thus improving mitochondrial respiration. Any disease process associated with poor energy delivery - Chronic fatigue syndrome, heart disease, dementia possibly cancer From Mitochondrial Function to Neuroprotection-an Emerging Role for Methylene Blue - MB can reroute electrons in the mitochondrial electron transfer chain directly from NADH to cytochrome c, increasing the activity of complex IV and effectively promoting mitochondrial activity while mitigating oxidative stress Low dose MB also acts as an antioxidant in mitochondria. MB interacts with oxygen to form water, which would decrease the superoxide radicals produced during the process of oxidative phosphorylation.
Potential for use in stroke, global cerebral ischemia, Alzheimer's disease, Parkinson's disease, and traumatic brain injury From Mitochondrial Function to Neuroprotection-an Emerging Role for Methylene Blue In both in vitro and in vivo studies, MB has shown impressive efficacy in mitigating neurodegeneration and the accompanying behavioural phenotypes in animal models for such conditions as stroke, global cerebral ischemia, Alzheimer's disease, Parkinson's disease, and traumatic brain injury.
Methylene Blue: Revisited It has been shown to attenuate the formations of amyloid plaques and neurofibrillary tangles and partial repair of impairments in mitochondrial function and cellular metabolism.
Chronic fatigue syndrome – due to its effect on mitochondria and possibly through reducing cytokines Methylene blue in covid-19 The only drug known to inhibit the excessive production of reactive species and cytokines is methylene blue, a low-cost dye with antiseptic properties used effectively to treat malaria, urinary tract infections, septic shock, and methemoglobinemia.
1880s Nobel laureate Paul Ehrlich discovered Methylene Blue as a malaria treatment, and it is considered safe and effective. Current studies have been investigating whether MB treatment alongside other antiparasitic drugs could prevent the parasites from developing drug resistance - see Efficacy and safety of methylene blue in the treatment of malaria: a systematic review

Photodynamic therapy using the light-activated antimicrobial agent, MB kills methicillin-resistant Staphylococcus aureus (MRSA) in superficial and deep excisional wounds - see Methylene Blue: Revisited

Any chronic infection may be susceptible to MB.

It is biologically plausible that combining MB with red light (ideally at a wavelength of 670nm – this activates MB) many potentiate the antimicrobial action of each other.

Methylene Blue: Revisited Photodynamic therapy using the light activated anti-microbial agent, Methylene blue kills methicillin resistant staphylococcus aureus (MRSA) in superficial and deep excisional wounds.Methylene blue in combination with light also inactivates viral nucleic acid of hepatitis-C and human immunodeficiency virus (HIV-1) and treats cases of resistant plaque psoriasis.
MB in combination with light (this is called photodynamic therapy) also inactivates viral nucleic acid of hepatitis-C and human immunodeficiency virus (HIV-1), Zika, Ebola, West Nile, Middle East respiratory syndrome, and treats cases of resistant plaque psoriasis - see Methylene Blue: Revisited Anti- viral Methylene Blue has a potent antiviral activity against SARS-CoV-2 and H1N1 influenza virus in the absence of UV-activation in vitro Our work supports the interest of testing methylene blue in clinical studies to confirm a preventive and/or therapeutic efficacy against both influenza virus H1N1 and SARS-CoV-2 infections.
Evidence suggests MB is effective against all the herpes viruses including herpes 1 and 2, EBV, CMV and VCZ.Effective against covid 19 (hydroxychloroquine, of proven benefit, is derived from methylene blue) See this US patent application for more details -US PATENT - METHYLENE BLUE THERAPY OF VIRAL DISEASE
MB is an antifungal agent and may inhibit candida by causing mitochondrial dysfunction in this species. Anti-fungal See Antifungal Action of Methylene Blue Involves Mitochondrial Dysfunction and Disruption of Redox and Membrane Homeostasis in C. albicans
Methylene blue (MB) binds to methaemoglobin and converts it to a more efficient form, thereby improving the symptoms of methemoglobinemia - see Recommendations for diagnosis and treatment of methemoglobinemia Carbon monoxide poisoning See Top 6 Benefits of Methylene Blue
MB is a monoamine oxidase inhibitor Depression – the dose used is very small – just 15mg per day A controlled trial of methylene blue in severe depressive illness Methylene blue, 15 mg/day, was compared with placebo in treatment of severe depressive illness. The 3-week trial was designed to avoid bias by placebo response and also to avoid observer bias. Improvement in patients receiving methylene blue was significantly greater than in those receiving placebo. Methylene blue at a dose of 15 mg/day appears to be a potent antidepressant, and further clinical evaluation is essential.

Dose of MB

MB should always be taken in low dose as high dose may have an opposite effect. The safe low dose is 1-2mg per Kg of body weight. Regardless of body weight start with 10mgs per day.

(It is so safe that it can be used directly into a vein, but doses of 15mgs/kg of body weight may cause haemolysis)

  1. Start with 1ml per day in two doses. Take this by putting drops into a small glass of water. Drink the blue solution. Your teeth may be temporarily stained blue, but this soon disappears. You may also pee blue!
  2. Increase the dose weekly in 10mgs ie 1ml increments
  3. A 60kg person could end up on 6ml per day for 1mgs per Kg, or 12ml per day for 2mgs/kg

UPDATE JUNE 2023 - I suggest you also add one to two grams of ascorbic acid – this converts much of the MB to leucoMB which is colourless. This may take several minutes but you get round the tooth/tongue staining issue. Drink the pale blue solution. In the body MB alternates between MB and leuco MB as it donates or accepts electrons. This explains the many desirable actions of MB. Expect still to pee blue. END OF UPDATE

Rather than buying as a liquid, it is more economical to purchase MB as the pure powder and dilute it yourself. Make sure this is British Pharmaceutical standard (it will have BP 73 on it ie the standards set in 1973) It may also be marked “Harmful if swallowed” – that simply reflects the fact that the powder is pure and needs to be diluted with water.

I purchased 10 grams (10,000mgs). 10 grams dissolved in 1,000ml (one litre) of water gives a 1% solution, for a daily dose of 120mgs (2mg per Kg) or 12ml this would last 80 days. See My Online Sales Website - Methylene Blue powder - 10g

Orthomolecular Medicine News Service Article on Methylene Blue - 4 February 2023

Please see Resolving Colds to Advanced COVID with Methylene Blue complete with 129 references.

Background Reading

See - The ultimate guide to methylene blue Mark Sloan

Concern about levels of Arsenic [As] and Lead [Pb]

Some website readers and patients have expressed concern about this. Good for them! I like it when people question me. It's what learning is all about but there is nothing to worry about here.

The APC specification for Methylene Blue BP73 is as follows: [Reference - APC specification for Methylene Blue BP73]


Minimum Assay: >99.5%
Molecular Formula: C16H18N3S
Molecular Weight: 373.90 g/mol
Melting Point: 190 deg C
Arsenic (As): <8ppm
Iron (Fe): <100ppm
Lead (Pb): <20ppm
Zinc (Zn): Passes Test
Loss on Drying: <10%

So, the calculations for the dose recommended here is [for As]

  1. MB has (<8) ppm of As. So, there are 8mgs As per kg MB
  2. We put 10grams MB in a litre and so we are putting 1/100 x 8mgs As in that litre
  3. That litre therefore contains 0.08mgs As
  4. The daily dose is, say, 10ml and so that 10ml will contain 1/100 x 0.08mgs of As
  5. The daily dose of MB contains 0.0008mgs As - inconsequential.

'Inconsequential'? Well yes, when you consider these figures regarding certain food types:


Similar calculations can be made for Lead. Also, having good levels of trace elements (Zn, Mg, Se etc), as Groundhog users will, will protect further.


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